Journal: Immunity

Article Title: CD8 + T cell activation in cancer comprises an initial activation phase in lymph nodes followed by effector differentiation within the tumor

doi: 10.1016/j.immuni.2022.12.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-human CD141 (FITC) , Miltenyi Biotec , clone: REA674, RRID: AB_2751167.

Techniques: Blocking Assay, Recombinant, Staining, Selection, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Hematology & Oncology

Article Title: BCMA-targeted immunotherapy for multiple myeloma

doi: 10.1186/s13045-020-00962-7

Figure Lengend Snippet: BCMA-targeted CAR-T cell products in clinical trials

Article Snippet: P-BCMA-101 , Poseida Therapeutics , Fully human anti-BCMA Centyrin TM , 4-1BB , PiggyBac™ (PB) DNA Modification System , No.

Techniques: Transduction, Modification

Journal: Cancers

Article Title: Expression of Tryptophan 2,3-Dioxygenase in Metastatic Uveal Melanoma

doi: 10.3390/cancers12020405

Figure Lengend Snippet: Immunohistochemical (IHC) staining of liver metastasis specimens. Liver metastasis specimens were stained for TDO, HMB45, and IDO. Positive cells show a distinct red stain. IHC staining of two representative sets of archived liver metastasis specimens were shown. ( A , D ) TDO staining: For negative control, TDO antibody was blocked with specific blocking peptide (a,d). Magnification: ×100 (left panels); ×200 (right panels) Scale bar = 100 μm. ( B , E ) HMB45 staining: Melanoma metastasis is positive (right bottom) and surrounding liver tissue is negative (right top). Isotype-matched IgG was used as negative control (b,e). Magnification: ×100 (left panels); ×200 (right panels) Scale bar = 100 μm ( C , F ) IDO staining: IDO is negative for both metastatic melanoma and surrounding liver tissue (top). Isotype-matched IgG is used as negative control (c,f). Magnification: ×100. Scale bar = 100 μm.

Article Snippet: The membrane was incubated with primary antibodies at 4 °C overnight: mouse anti-human TDO antibody purchased from Abnova (#H00006999-B01P; dilution 1:500, San Jose, CA, USA), anti-human IDO antibody (#D5J4E TM ; dilution 1:1000) and anti-human β-actin antibody (#D6A8; dilution 1:10,000) purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Negative Control, Blocking Assay

Journal: Cancers

Article Title: Expression of Tryptophan 2,3-Dioxygenase in Metastatic Uveal Melanoma

doi: 10.3390/cancers12020405

Figure Lengend Snippet: Expressions of TDO and IDO in MUM cell lines. ( A ) IDO1 and TDO2 mRNA expressions were evaluated by RT-PCR. GAPDH was used as a loading control. ( B ) TDO and IDO protein expression in MUM cell lines. β–actin was used as a loading control.

Article Snippet: The membrane was incubated with primary antibodies at 4 °C overnight: mouse anti-human TDO antibody purchased from Abnova (#H00006999-B01P; dilution 1:500, San Jose, CA, USA), anti-human IDO antibody (#D5J4E TM ; dilution 1:1000) and anti-human β-actin antibody (#D6A8; dilution 1:10,000) purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing